Background: Onchocerciasis, a severely debilitating neglected tropical disease caused by Onchocerca volvulus, has been earmarked for global elimination by the year 2040. To achieve the elimination targets, new tools such as adulticidal drugs, vaccine candidates and diagnostics are urgently needed. With the recent elucidation of the entire genome of O. volvulus comes a great opportunity for research on parasitic enzymes which may be important players in the parasite’s survival. Previous researchers have identified 16 top genes that code for possible chokepoint enzymes. These 16 hypothetical proteins are possible targets of repurposed drugs and could offer a cheaper alternative to developing drugs from scratch. However, these enzymes have not been molecularly confirmed in the parasites and their biochemical character as yet unknown. The study will use immunohistochemistry and microscopy to determine the presence and anatomic locations of amidase enzyme and then clone, overexpress and purify the enzymes. Enzyme activity, substrate specificity assays and kinetic analysis will be performed to provide insight on the nature of the enzyme. It is expected that this study will provide insight into the parasites’ metabolism and demonstrate the utility of amidase as therapeutic targets with future prospects as vaccine candidates and/or diagnostic markers.